Separation of the coproporphyrin isomers I and III by thin layer chromatography.

Excretion pattern of faecal coproporphyrin isomers I-IV in human porphyrias.

The relative proportions of the four coproporphyrin isomers I-IV were analysed in faeces of 20 healthy subjects and 60 patients suffering from one of the seven common types of hepatic or erythropoietic hereditary porphyrias. A newly developed, reliable method for sample preparation was applied, using reversed-phase thin layer chromatography for the isolation of naturally occurring coproporphyri...

High-performance liquid chromatography of coproporphyrin isomers.

A reversed-phase system is described for the simultaneous isocratic separation of coproporphyrin I, II, III and IV isomers. The retention behaviour of coproporphyrin I and III is studied in detail. The method is suitable for both analytical and semi-preparative separation.

Determination of coproporphyrin I and III isomers by high-performance liquid chromatography.

Unidimensional Thin Layer Chromatography of Salicylic Acid Derivatives on Boric Acid-Impregnated Plates in Drugs Studies

A unidimensional Thin Layer Chromatography system for the separation of salicylic acid and its derivatives (sulfosalicylic acid and Aspirin) has been developed. The separation was successfully using a solvent system of chloroform-methanol-water-ammonium hydroxide 120:75:6:2 (V/V) as mobile phase on TLC plates impregnated with boric acid. The method is based on different behavior of investigated...

Quantification of urinary porphyrins by liquid chromatography after oxidation of porphyrinogens.

A quantitative "high-performance" liquid-chromatographic method is described for determining porphyrins in human urine. Porphyrinogens in urine are first converted to the corresponding porphyrins by oxidation with iodine. Uroporphyrin, hepatacarboxylic acid porphyrin, hexacarboxylic acid porphyrin, pentacarboxylic acid porphyrin, and coproporphyrin I and III isomers are then separated on a reve...